Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ) (CAT#: HPAB-0440-YJ)
This product is a recombinant Mouse antibody that recognizes CD19 scFv (FMC63). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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SDS-PAGE
Figure 1 Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ) in SDS-PAGE
Lane 1: Non-reduced mAb
Lane 2: Reduced mAb
SEC-HPLC
Figure 2 Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ) in HPLC
The purity of HPAB-0440-YJ was greater than 95% as determined by SEC-HPLC.
SDS-PAGE
Figure 3 Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ, Lot#CB0822JF05) in SDS-PAGE
SDS-PAGE analysis of HPAB-0440-YJ in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of HPAB-0440-YJ was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 4 Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ, Lot#CB0822JF05) in HPLC
The purity of HPAB-0440-YJ was greater than 95% as determined by SEC-HPLC.
ELISA
Figure 5 Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ, Lot#CB0822JF05) in ELISA
ELISA analysis of HPAB-0440-YJ was performed by coating with FMC63 antibody.
HPAB-0440-YJ is labeled with biotin and detected by HRP-SA
SDS-PAGE
Figure 6 Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ)-Fc silenced in SDS-PAGE
SDS-PAGE analysis of HPAB-0440-YJ, Fc silenced, in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.
SEC-HPLC
Figure 7 Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ)-Fc silenced in SEC-HPLC
The purity of HPAB-0440-YJ, Fc silenced, was greater than 98% as determined by SEC-HPLC.
WB
Figure 8 Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ)-Fc silenced in WB
Western blot analysis of HPAB-0440-YJ, Fc silenced, was performed by loading HPAB-0440-YJ-FcS as antigen. TAB-1611CL-F(E) as antibody.
Specifications
- Immunogen
- L Cells expressing CD19scFvmCD8a
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Mouse antibody scFv (FMC63)
- Species Reactivity
- Mouse
- Applications
- ELISA, FC, WB, Inhib, IHC
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD19 scFv; FMC63
Related Resources
Gravina, Alessia, et al. "Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression." Nature biotechnology 41.5 (2023): 717-727. https://doi.org/s41587-022-01540-7
The study investigates the protection of cell therapeutics from antibody-mediated killing through the overexpression of CD64. This approach enhances the survival and efficacy of allogeneic cell therapeutics used in cancer therapy and regenerative medicine. The research demonstrates that engineered cells overexpressing CD64 can effectively evade antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), which are primary mechanisms of antibody-mediated rejection.
At Creative Biolabs, we provided several essential reagents for this research. These included the humanized anti-MICA IgG1 antibody (Cat#: TAB-0799CL), the humanized anti-CD52 IgG1 antibody (Cat#: FAMAB-0089WJ), the humanized anti-CD52 IgG1 antibody (Cat#: FAMAB-0014JF), and the hypusine antibody (Cat#: HPAB-0440-YJ). Our products were crucial for the experiments involving antibody-mediated killing assays, enabling the detailed examination of how engineered cells can evade immune detection and destruction. This study highlights the potential of CD64 overexpression to enhance the viability of cell-based therapies, underscoring our commitment to supporting advanced research and innovative therapeutic solutions.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0440-YJ-S(P) | Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody; scFv Fragment (HPAB-0440-YJ-S(P)) | ELISA, FC, WB, Inhib, IHC | Mouse scFv |
Customer Reviews and Q&As
Customer Review
Q&As
Michael King
Excellent for Targeting CD19
Excellent for Targeting CD19
The Anti-CD19 scFv (FMC63) Recombinant Antibody has delivered excellent results in our flow cytometry and immunoassays. The specificity and sensitivity for CD19 are top-notch, providing clear and accurate detection. This antibody has greatly improved our targeting studies and made our experiments more reliable.
22.Mar,23
Rachel Adams
High Specificity and Sensitivity
High Specificity and Sensitivity
We incorporated this antibody into our Western blot and immunoprecipitation assays, and it has been a great addition. The specificity and sensitivity for CD19 are outstanding, consistently detecting the target protein with high clarity. The reliable performance has made it an essential tool in our research.
19.Nov,22
John Harris
Reliable in Multiple Assays
Reliable in Multiple Assays
This antibody has proven to be incredibly reliable in various assays, including flow cytometry and immunofluorescence. The high-quality, reproducible results and strong affinity for CD19 have significantly benefited our experiments, making it a staple in our lab. The consistent performance has greatly enhanced our data quality.
11.Jun,21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0440-YJ, RRID: AB_3111397)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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