Mouse Anti-CD19 scFv (FMC63) Recombinant Antibody (HPAB-0440-YJ)

Customer Review

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Michael King
22.Mar,23

Excellent for Targeting CD19

The Anti-CD19 scFv (FMC63) Recombinant Antibody has delivered excellent results in our flow cytometry and immunoassays. The specificity and sensitivity for CD19 are top-notch, providing clear and accurate detection. This antibody has greatly improved our targeting studies and made our experiments more reliable.

Rachel Adams
19.Nov,22

High Specificity and Sensitivity

We incorporated this antibody into our Western blot and immunoprecipitation assays, and it has been a great addition. The specificity and sensitivity for CD19 are outstanding, consistently detecting the target protein with high clarity. The reliable performance has made it an essential tool in our research.

John Harris
11.Jun,21

Reliable in Multiple Assays

This antibody has proven to be incredibly reliable in various assays, including flow cytometry and immunofluorescence. The high-quality, reproducible results and strong affinity for CD19 have significantly benefited our experiments, making it a staple in our lab. The consistent performance has greatly enhanced our data quality.

Q&As

1. What is the specificity of the anti-CD19 scFv FMC63 recombinant antibody in flow cytometry?

   A: The anti-CD19 scFv FMC63 recombinant antibody (HPAB-0440-YJ) is highly specific for CD19. It provides reliable detection of CD19-expressing cells in flow cytometry assays, making it a valuable tool for immunophenotyping and other studies involving CD19.

2. Is this anti-CD19 scFv FMC63 recombinant antibody suitable for use in immunohistochemistry?

   A: Yes, the anti-CD19 scFv FMC63 recombinant antibody (HPAB-0440-YJ) is suitable for use in immunohistochemistry. It can specifically bind to CD19 in tissue sections, allowing for the visualization of CD19-expressing cells in histological samples.

3. What is the recommended concentration for using the anti-CD19 scFv FMC63 recombinant antibody in Western blot?

   A: The recommended concentration for using the anti-CD19 scFv FMC63 recombinant antibody (HPAB-0440-YJ) in Western blot is typically 1-2 µg/mL. It is advisable to optimize the concentration based on the specific experimental conditions and sample types to achieve the best results.

4. Can the anti-CD19 scFv FMC63 recombinant antibody be used in flow cytometry to sort CD19+ cells?

   A: Yes, the anti-CD19 scFv FMC63 recombinant antibody (HPAB-0440-YJ) can be used in flow cytometry to sort CD19+ cells. It is specifically designed to bind to CD19, making it effective for isolating CD19-expressing cells from mixed cell populations.

5. What are the recommended storage conditions for the anti-CD19 scFv FMC63 recombinant antibody?

   A: The recommended storage condition for the anti-CD19 scFv FMC63 recombinant antibody (HPAB-0440-YJ) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Gravina, Alessia, et al. "Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression." Nature biotechnology 41.5 (2023): 717-727. https://doi.org/s41587-022-01540-7
   The study investigates the protection of cell therapeutics from antibody-mediated killing through the overexpression of CD64. This approach enhances the survival and efficacy of allogeneic cell therapeutics used in cancer therapy and regenerative medicine. The research demonstrates that engineered cells overexpressing CD64 can effectively evade antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), which are primary mechanisms of antibody-mediated rejection.
   At Creative Biolabs, we provided several essential reagents for this research. These included the humanized anti-MICA IgG1 antibody (Cat#: TAB-0799CL), the humanized anti-CD52 IgG1 antibody (Cat#: FAMAB-0089WJ), the humanized anti-CD52 IgG1 antibody (Cat#: FAMAB-0014JF), and the hypusine antibody (Cat#: HPAB-0440-YJ). Our products were crucial for the experiments involving antibody-mediated killing assays, enabling the detailed examination of how engineered cells can evade immune detection and destruction. This study highlights the potential of CD64 overexpression to enhance the viability of cell-based therapies, underscoring our commitment to supporting advanced research and innovative therapeutic solutions.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-0440-YJ, RRID: AB_3111397)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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